Coding

Part:BBa_K1051500:Design

Designed by: Lin Qiongfen   Group: iGEM13_Shenzhen_BGIC_ATCG   (2013-07-02)


Sic1 mutation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 310


Design Notes

Mutate seven phosphorylation site of Sic1 and one site of Spe1's restriction site.



Source

Come from saccharomyces cerevisiae.


References